| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | GPRchr2; WI31133 |
| Description | Human GPR75-Strep full length protein-synthetic nanodisc |
| Delivery | 6~8weeks |
| Uniprot ID | O95800 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length GPR75-Strep protein has a MW of 59.4 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human GPR75-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag GPR75-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-GPR75 monoclonal antibody (DMC100368) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Strep-Nanodisc is 15.14ng/ml.
Figure 2. Human GPR75-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
Figure 3. WB analysis of GPR75-Strep-Nanodisc with anti-GPR75 monoclonal antibody (DMC100368), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
Figure 4. Loaded Human GPR75 monoclonal antibody (DMC100368) on Pro-A Biosensor, can bind human GPR75-Strep full length protein-synthetic nanodisc with an affinity constant of 5.02nM as determined in SPR assay.
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